Application

Research Sub CategoryIntegrins

Anti-Integrin β2 [CD18] Antibody detects level of Integrin β2 [CD18] & has been published & validated for use in FC, WB, IH.

Research CategoryCell Structure

Immunohistochemistry: Identification of CD18+ leucocytes on cryostat sections

Function Blocking: Studies of cell-cell and cell-matrix adhesion, this antibody will block cell-cell interaction

Flow Cytometry

Western Blot

Optimal working dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Leucocytes from an LGL - type leukaemia

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: CBL514

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

The monoclonal is presented in phosphate buffered saline containing 15mM sodium azide, pH 7.4. We recommend that each laboratory determine an optimum working titre for use in its particular application.

Specificity

This antibody recognizes the CD18 molecule, a 95 kDa integrin molecule (beta 2 integrin) that forms the common beta chain of the CD11/CD18 heterodimer integrins which are expressed on all leucocytes. The beta2 integrin is the ICAM 1 receptor

FUSION PARTNER:

Sp 2/0 - Ag 14 myeloma cell line

Storage and Stability

Maintain at 2o-8oC for up to 12 months from date of receipt. For long term storage aliquot antibody into small volumes and store at -20°C for up to one year.